Journal: BMC Biotechnology

Article Title: Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiae harbouring xylose isomerase

doi: 10.1186/1472-6750-14-41

Figure Lengend Snippet: Comparison of parental and mutated strain in batch culture. Batch growth profiles for the S. cerevisiae parental strain D5A + (squares) and mutant strain D5A + (circles) in water-diluted (67% v/v, solid line) and undiluted (broken line) liquor from steam-pretreated triticale supplemented with 20 g xylose/L in shake flask cultures. Culture growth was quantified as total cell counts using a counting chamber. Data represents the average values from duplicate counts.

Article Snippet: The authors gratefully acknowledge Dr. L. Favaro Prof. S. Casella and Prof. M. Basaglia from Department of Agronomy Food Natural resources Animals and Environment (DAFNAE) University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro (PD), Italy for providing S. cerevisiae strain MEL2, used as benchmark in this study.

Techniques: Comparison, Mutagenesis, Serial Time-encoded Amplified Microscopy

Journal: BMC Biotechnology

Article Title: Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiae harbouring xylose isomerase

doi: 10.1186/1472-6750-14-41

Figure Lengend Snippet: Batch culture profiles after hardening with xylose as carbon source. Batch culture profiles for the recombinant xylose-utilising S. cerevisiae strains D5A + (parental strain, A and B ), TMB3400 (C and D) and D5A +H (hardened strain, E and F ) in a growth medium supplemented with 50% (v/v) pretreatment liquor from steam-pretreated triticale and 20 g xylose/L in shake flask cultures. Figure symbols left-hand column: biomass (circles), ethanol (diamonds), xylose (triangles). Figure symbols right-hand column: acetic acid (squares), formic acid (circles), HMF (diamonds) and furfural (crosses). Error bars represent standard deviations from duplicate experiments.

Article Snippet: The authors gratefully acknowledge Dr. L. Favaro Prof. S. Casella and Prof. M. Basaglia from Department of Agronomy Food Natural resources Animals and Environment (DAFNAE) University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro (PD), Italy for providing S. cerevisiae strain MEL2, used as benchmark in this study.

Techniques: Recombinant, Serial Time-encoded Amplified Microscopy

Journal: BMC Biotechnology

Article Title: Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiae harbouring xylose isomerase

doi: 10.1186/1472-6750-14-41

Figure Lengend Snippet: Growth parameters with glucose as carbon source

Article Snippet: The authors gratefully acknowledge Dr. L. Favaro Prof. S. Casella and Prof. M. Basaglia from Department of Agronomy Food Natural resources Animals and Environment (DAFNAE) University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro (PD), Italy for providing S. cerevisiae strain MEL2, used as benchmark in this study.

Techniques:

Journal: BMC Biotechnology

Article Title: Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiae harbouring xylose isomerase

doi: 10.1186/1472-6750-14-41

Figure Lengend Snippet: Batch culture profiles after hardening with glucose as carbon source. Batch cultivation profiles for S. cerevisiae strains D5A +H (A and B) , TMB3400 (C and D) , MH1000 (E and F) and MEL2 (G and H) in 50% (v/v) triticale pretreatment liquor supplemented with 20 g glucose/L in shake flask cultures. Figure symbols: biomass (circles), glucose (triangles), ethanol (diamonds), acetic acid (squares), formic acid (circles). Error bars represent standard deviations from duplicate experiments.

Article Snippet: The authors gratefully acknowledge Dr. L. Favaro Prof. S. Casella and Prof. M. Basaglia from Department of Agronomy Food Natural resources Animals and Environment (DAFNAE) University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro (PD), Italy for providing S. cerevisiae strain MEL2, used as benchmark in this study.

Techniques:

Journal: BMC Biotechnology

Article Title: Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiae harbouring xylose isomerase

doi: 10.1186/1472-6750-14-41

Figure Lengend Snippet: Fed-batch SSF culture using steam-pretreated sweet sorghum bagasse. Single fed-batch SSF experiments of pressed solids fed-batch using S. cerevisiae strains D5A +H (A) and TMB 3400 (B) . Figure symbols: ethanol (diamonds), glucose (circles) and xylose (triangles). Arrows indicate the addition of substrate in 2.5% (g/v) increments at 0, 24, 48 and 72 h.

Article Snippet: The authors gratefully acknowledge Dr. L. Favaro Prof. S. Casella and Prof. M. Basaglia from Department of Agronomy Food Natural resources Animals and Environment (DAFNAE) University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro (PD), Italy for providing S. cerevisiae strain MEL2, used as benchmark in this study.

Techniques: Serial Time-encoded Amplified Microscopy

Journal: bioRxiv

Article Title: Resistance to the antifungal activity of Aprotinin occurs through mutations in genes that function in cation homeostasis

doi: 10.1101/2020.06.22.164863

Figure Lengend Snippet: (A) BPTI-resistant strains were more tolerant to sodium chloride, calcofluor white and hydrogen peroxide than wildtype cells. BPTI-resistant and wild-type S. cerevisiae BY4741 cells were diluted and spotted out on to YPD Agar containing different concentrations of abiotic stressors. A no treatment control was prepared using the same cell preparations and at the same time as the treatment plates. The three BPTI-resistant strains grew better than wildtype in the presence of 300mM of NaCl BPTI-resistant strains were more resistant to 5μg/mL CFW compared to wild type. BPTI-resistant strain B grew better than wildtype in the presence of 5mM hydrogen peroxide whereas BPTI-resistant strains A and C had similar growth to wildtype. Images are representative of three replicate experiments. (B) BPTI-resistant strains were more tolerant to Hexamine (III) cobalt chloride than wildtype. Growth inhibition of BPTI-resistant strains by Hexamine (III) cobalt chloride (HCC) relative to wildtype control. BPTI-resistant strains were more tolerant to the magnesium channel inhibitor HCC with an MIC of 10 μM compared to wild type at 2.5 μM. Growth % is relative to the highest measured absorbance for each strain and the wild-type S. cerevisiae BY4741. Error bars represent +/− standard error of the mean (n= 3). (C) Cell growth of BPTI-resistant strains in YPD media compared to wild-type S. cerevisiae BY4741. There was no difference in growth of the BPTI-resistant strains in YPD compared to wildtype.

Article Snippet: The S. cerevisiae strain BY4741 (MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0) was purchased from Thermo Scientific.

Techniques: Control, Inhibition

Journal: bioRxiv

Article Title: Resistance to the antifungal activity of Aprotinin occurs through mutations in genes that function in cation homeostasis

doi: 10.1101/2020.06.22.164863

Figure Lengend Snippet: (left) No treatment control plate that was incubated at the same time as the treatment plates. (middle) BPTI-resistant and wild-type S. cerevisiae BY4741 cells were diluted and spotted out on to YPD agar, before exposure to UV for 5min. There was no difference in sensitivity between the BPTI-resistant strains, control plate, and wild type, when exposed to UV. (right) There is no difference in sensitivity of the BPTI-resistant strains to SDS compared to the controls and wild type.

Article Snippet: The S. cerevisiae strain BY4741 (MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0) was purchased from Thermo Scientific.

Techniques: Control, Incubation